Journal: Scientific Reports
Article Title: Effects of proximal tubule-specific ATRAP enhancement on hypertension in a remnant kidney chronic kidney disease model of mice
doi: 10.1038/s41598-025-12168-3
Figure Lengend Snippet: Expression and localization of angiotensin II type 1 receptor-associated protein (ATRAP) in proximal tubule-specific ATRAP transgenic (PT-Tg) and littermate-control (LC) mice. (a) Representative western blots and quantitative analysis of ATRAP in the kidney of littermate-control (LC) and proximal tubule-specific ATRAP transgenic (PT-Tg) mice. Values are expressed as mean ± SEM (n = 5 in each group). *** P < 0.001 versus LC, unpaired t -test. (b) Renal cortical sections showing ATRAP expression in renal tubules detected by anti-ATRAP antibody (left panels). Consecutive sections were stained with a monoclonal antibody against megalin (middle panels), a specific marker of proximal tubule (PT), and a monoclonal antibody against calbindin-D (right panels), a specific marker of distal convoluted tubule (DT) and connecting tubule. Original magnification: × 400. (c, d, e) Quantitative analysis of calbindin-D, megalin and ATRAP mRNA expression in Glo, PTs, and DTs of the renal cortex identified by the LMD method. Values are normalized relative to 18S rRNA control levels. Values are expressed as mean ± SEM (n = 4 mice in mix). ATRAP, Angiotensin II type 1 receptor-associated protein; LC, littermate-control; PT-Tg, proximal tubule-specific ATRAP transgenic; Glo, glomerulus; PT, proximal tubule; DT, distal tubule; LMD, Laser‐capture microdissection.
Article Snippet: Samples were run on a 5–20% polyacrylamide gel, transferred to PVDF membranes, blocked, and probed with specific primary antibodies against ATRAP (diluted 1:2000, developed in our laboratory as previously described – ), HA (diluted 3:2000, A190-108A-2; Bethyl Laboratories, Montgomery, TX, USA) and GAPDH (diluted 1:2000, GTX100118; GeneTex, Irvine, CA, USA).
Techniques: Expressing, Transgenic Assay, Control, Western Blot, Staining, Marker, Laser Capture Microdissection
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